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KMID : 0358419950380101909
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Korean Journal of Obstetrics and Gynecology
1995 Volume.38 No. 10 p.1909 ~ p.1923
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Regulation of Insulin-like Growth Factor-Binding Proteins Production in Human Luteinizing Granulosa Cells
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Abstract
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In order to study the regulation of insulin-like growth factor-binding proteins(IGFBPs) production by human luteinizing granulosa cells, cells from human prevulatory follicles were cultured under serum-free conditions in the presence or absence
of
protein kinase-C(PKC) activator, 12-0-tetradecanoyl phorbol-13-acetate(TPA), adenylate cyclase activators, follicle stimulationg hormone(FSH), human chorionic gonadotropin(hCG), 3'5'-cyclic monophosphate (cAMP) and human growth hormone(GH).
IGFBP-3
and
IGFBP-2 were consistently detected in control conditioned media by western ligand blotting and immunoprecipitation but IGFBP-4 and IGFBP-1 were variable present. IGFBP-3 and IGFBP-2 production by luteinizing granulosa cells were inhibited by
hCG(0.1-10㎍/ml). FSH and c-AMP affected IGFBPs production in a manner different from hCG. Treatment of luteinizing granulosa cells cultrues with TPA(0.1~1.0ng/ml ) resulted in significant increase in IGFBP-3 production while contrary effects on
IGFBP-1
production were observed. GH(10~1,000ng/ml) significantly stimulated production of IGFBP-3 and IGFBP-2 compared with control.
Our data indicate that the production of IGFBP-3 and IGFBP-2 by human luteinizing granulosa cells may be regulated by both PKC and adenylate cyclase pathway and that the type of adenylate cyclase activators may affect IGFBPs production human
luteinizing
granulosa cells.
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